Plasmid

Part:BBa_K277000:Design

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-19)

pGEM-T Cloning Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NotI site found at 11
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NgoMIV site found at 2640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1412
    Illegal SapI site found at 329


Design Notes

This backbone was purchased from Promega and is compatible with RFC 38

Source

Promega Corporation

References